PPP1CB Rekombinanter Antikörper

PPP1CB Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242547H7

Kat-Nr. : 84938-5-PBS

Synonyme

242547H7, EC:3.1.3.16, PP 1B, PP1 Beta, PP-1B

---

• 

  WB analysis using 84938-5-RR (same clone as 84938-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84938-5-RR (PPP1CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84938-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84938-5-RR (same clone as 84938-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84938-5-RR (PPP1CB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84938-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84938-5-RR (same clone as 84938-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84938-5-RR (PPP1CB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84938-5-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 84938-5-RR (same clone as 84938-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PPP1CB antibody (84938-5-RR, Clone: 242547H7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84938-5-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 84938-5-RR (same clone as 84938-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP1CB antibody (84938-5-RR, Clone: 242547H7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84938-5-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 84938-5-RR (same clone as 84938-5-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PPP1CB Recombinant antibody (84938-5-RR, Clone:242547H7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84938-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84938-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84938-5-RR against Human PPP1CB were performed. The affinity constant is 2.44 nM.

---

Geprüfte Anwendungen

Produktinformation

84938-5-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA PPP1CB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PPP1CB fusion protein Ag0183
• Vollständiger Name: protein phosphatase 1, catalytic subunit, beta isoform
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC002697
• Gene symbol: PPP1CB
• Gene ID (NCBI): 5500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates.