PPP1R10 Polyklonaler Antikörper

PPP1R10 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (3)

Kat-Nr. : 24450-1-AP

Synonyme

CAT53, FB19, p99, PNUTS, PP1 binding protein of 114 kDa, PP1R10, PPP1R10, Protein FB19

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• 

  WB analysis of HepG2 using 24450-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24450-1-AP (PPP1R10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 24450-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24450-1-AP (PPP1R10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

24450-1-AP bindet in WB, IF/ICC, IP, ELISA PPP1R10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP1R10 fusion protein Ag20091
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 10
• Berechnetes Molekulargewicht: 940 aa, 99 kDa
• Beobachtetes Molekulargewicht: 99 kDa
• GenBank-Zugangsnummer: BC150310
• Gene symbol: PPP1R10
• Gene ID (NCBI): 5514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Nat Cell Biol; A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression.; Authors - Simon Grelet
human	WB,IP	J Exp Clin Cancer Res; Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer; Authors - Macarena Morillo-Huesca

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lucas (Verified Customer) (04-15-2025)

Works ok in Western Blot, it seems to depend on the secondary antibody used.

• Applications: Western Blot

FH

Roy (Verified Customer) (06-15-2024)

Perfect to detect PNUTS by WB (1/1000, Overnight incubation at 4°C) and by IF (1/250) in HeLa cells

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

Fabienne (Verified Customer) (06-11-2024)

Works well in western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cell line