PPP1R13L Rekombinanter Antikörper

PPP1R13L Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250633E12

Kat-Nr. : 86044-1-PBS

Synonyme

IASPP, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13B like protein

---

• 

  WB analysis using 86044-1-RR (same clone as 86044-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86044-1-RR (PPP1R13L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86044-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02334-1

  The mean iASPP concentration was determined to be 32.51 ng/mL in PC-3 cell extract based on a 2.0 mg/mL extract load and 68.98 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP02334-1

  Sandwich ELISA standard curve of MP02334-1, Human iASPP Recombinant Matched Antibody Pair - PBS only. 86044-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag13273. 86044-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

• 

  Affinity and Kinetic Characterization of 86044-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86044-1-RR against Human PPP1R13L were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

86044-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA PPP1R13L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PPP1R13L fusion protein Ag13273
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 13 like
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC064913
• Gene symbol: PPP1R13L
• Gene ID (NCBI): 10848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53-mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression.