PPP1R15B Polyklonaler Antikörper

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• KD/KO Validated

PPP1R15B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14634-1-PBS

Synonyme

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  WB analysis of SGC-7901 using 14634-1-AP (same clone as 14634-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14634-1-AP (same clone as 14634-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SGC-7901 using 14634-1-AP (same clone as 14634-1-PBS)

  WB result of PPP1R15B antibody (14634-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP1R15B transfected SGC-7901 cells. This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14634-1-AP (same clone as 14634-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14634-1-AP (same clone as 14634-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 14634-1-AP (same clone as 14634-1-PBS)

  IP result of anti-PPP1R15B (IP:14634-1-AP, 3ug; Detection:14634-1-AP 1:1000) with MCF-7 cells lysate 2500ug. This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14634-1-AP (same clone as 14634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14634-1-AP (PPP1R15B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 14634-1-AP (same clone as 14634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14634-1-AP (PPP1R15B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14634-1-AP (same clone as 14634-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 14634-1-AP (PPP1R15B antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG . This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 14634-1-AP (same clone as 14634-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP1R15B antibody (14634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14634-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14634-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PPP1R15B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP1R15B fusion protein Ag6213
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 15B
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC065280
• Gene symbol: PPP1R15B
• Gene ID (NCBI): 84919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).