PPP1R15B Rekombinanter Antikörper

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• KD/KO Validated

PPP1R15B Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240052G5

Kat-Nr. : 83016-6-PBS

Synonyme

PPP1R15B;CReP, 240052G5, CREP, FLJ14744

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  WB analysis using 83016-6-RR (same clone as 83016-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83016-6-RR (PPP1R15B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 83016-6-RR (same clone as 83016-6-PBS)

  WB result of PPP1R15B antibody (83016-6-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP1R15B transfected SGC-7901 cells. This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83016-6-RR (same clone as 83016-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PPP1R15B antibody (83016-6-RR, Clone: 240052G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83016-6-RR (same clone as 83016-6-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug PPP1R15B Recombinant antibody (83016-6-RR, Clone:240052G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83016-6-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA PPP1R15B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PPP1R15B fusion protein Ag6213
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 15B
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC065280
• Gene symbol: PPP1R15B
• Gene ID (NCBI): 84919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).