PPP1R8 Polyklonaler Antikörper

PPP1R8 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16115-1-PBS

Synonyme

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  WB analysis of PC-3 using 16115-1-AP (same clone as 16115-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16115-1-AP (same clone as 16115-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16115-1-AP (same clone as 16115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16115-1-AP (same clone as 16115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16115-1-AP (same clone as 16115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16115-1-AP (same clone as 16115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16115-1-AP (same clone as 16115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16115-1-AP (same clone as 16115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16115-1-AP (same clone as 16115-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16115-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16115-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PPP1R8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP1R8 fusion protein Ag9041
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 8
• Berechnetes Molekulargewicht: 209 aa, 23 kDa
• Beobachtetes Molekulargewicht: 47 kDa, 28 kDa, 19 kDa
• GenBank-Zugangsnummer: BC013360
• Gene symbol: PPP1R8
• Gene ID (NCBI): 5511
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP1R8, also named as ARD1, NIPP1, is a 351 amino acid protein, which has a basic N- and C-terminal and an acidic central domain. PPP1R8 is ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. PPP1R8 has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates and may also be involved in pre-mRNA splicing. PPP1R8 exists many isoforms and molecular weight of modified isoforms is about 18 kDa, 28 kDa and 47 kDa.