PPP2R2A Polyklonaler Antikörper

PPP2R2A Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16569-1-PBS

Synonyme

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  WB analysis using 16569-1-AP (same clone as 16569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16569-1-AP (PPP2R2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16569-1-AP (same clone as 16569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16569-1-AP (PPP2R2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16569-1-AP (same clone as 16569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16569-1-AP (PPP2R2A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16569-1-AP (same clone as 16569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16569-1-AP (PPP2R2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 16569-1-AP (same clone as 16569-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPP2R2A antibody (16569-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16569-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PPP2R2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2R2A fusion protein Ag9851
• Vollständiger Name: protein phosphatase 2 (formerly 2A), regulatory subunit B, alpha isoform
• Berechnetes Molekulargewicht: 477 aa, 52 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC041071
• Gene symbol: PPP2R2A
• Gene ID (NCBI): 5520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The serine/threonine protein phosphatase 2A (PP2A) is a master regulator of the complex cellular signaling that occurs during all stages of mammalian development. PPP2R2A, also known as B55A or PR55A, is a regulatory subunit of PP2A expressed in an Akt-dependent manner in epidermis during barrier formation. PPP2R2A participates in the negative control of cell growth and division. PPP2R2A has been proved to be a tumor suppressor that can inhibit the proliferation of a variety of cancer cells, such as prostate cancer cells(PMID: 21872824).