PPP2R2A/B/C Monoklonaler Antikörper

PPP2R2A/B/C Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E1D5

Kat-Nr. : 67783-1-PBS

Synonyme

PPP2R2B, PP2A subunit B isoform B55-beta, PP2A PR55B, PP2A B55BETA, B55β

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  WB analysis using 67783-1-Ig (same clone as 67783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  WB analysis using 67783-1-Ig (same clone as 67783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP2R2A/B/C antibody (67783-1-Ig, Clone: 2E1D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67783-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PPP2R2A/B/C und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PPP2R2A/B/C fusion protein Ag30774
• Vollständiger Name: protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• Berechnetes Molekulargewicht: 443 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC031790
• Gene symbol: PPP2R2B
• Gene ID (NCBI): 5521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PPP2R2B gene encodes a deduced 443 amino acid protein of approximately 52 kDa, which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody can recognize PPP2R2A and PPP2R2C due to the immunogen of PPP2R2B having high homology with PPP2R2A and PPP2R2C.