PPP2R2B/A/C/D Polyklonaler Antikörper

PPP2R2B/A/C/D Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13123-1-PBS

Synonyme

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  WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 13123-1-AP (same clone as 13123-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  IP experiment of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

  IP result of anti-PPP2R2B/A/C/D (IP:13123-1-AP, 4ug; Detection:13123-1-AP 1:500) with SH-SY5Y cells lysate 1200ug. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13123-1-AP (same clone as 13123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP2R2B/A/C/D antibody (13123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13123-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PPP2R2B/A/C/D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2R2B/A/C/D fusion protein Ag3860
• Vollständiger Name: protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• Berechnetes Molekulargewicht: 443 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC031790
• Gene symbol: PPP2R2B
• Gene ID (NCBI): 5521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody has cross-reactivity to PPP2R2A, PPP2R2C and PPP2R2D.