PPP3CA Monoklonaler Antikörper

PPP3CA Monoklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A5C1

Kat-Nr. : 68163-1-PBS

Synonyme

CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha

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  WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 68163-1-Ig (same clone as 68163-1-PBS)

  IP result of anti-PPP3CA (IP:68163-1-Ig, 5ug; Detection:68163-1-Ig 1:4000) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68163-1-PBS bindet in WB, IHC, IP, Indirect ELISA PPP3CA und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PPP3CA fusion protein Ag32642
• Vollständiger Name: protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC025714
• Gene symbol: PPP3CA
• Gene ID (NCBI): 5530
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.