PPP3CB Polyklonaler Antikörper

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• KD/KO Validated

PPP3CB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 13340-1-AP

Synonyme

CALNA2, Calmodulin-dependent calcineurin A subunit beta isoform, CNA beta, CAM-PRP catalytic subunit, CAM PRP catalytic subunit

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  WB analysis of HEK-293 using 13340-1-AP

  WB result of PPP3CB antibody (13340-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP3CB transfected HEK-293 cells.

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  WB analysis using 13340-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13340-1-AP (PPP3CB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13340-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13340-1-AP (PPP3CB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13340-1-AP

  IP result of anti-PPP3CB (IP:13340-1-AP, 4ug; Detection:13340-1-AP 1:2000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of mouse brain using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 13340-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PPP3CB antibody (13340-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, humanes Herzgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

13340-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PPP3CB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP3CB fusion protein Ag4163
• Vollständiger Name: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
• Berechnetes Molekulargewicht: 525 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC028049
• Gene symbol: PPP3CB
• Gene ID (NCBI): 5532
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
human	IF	Autophagy; SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis; Authors - Qin Xia
human	IP	Cell Death Differ; The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma.; Authors - Jialiang Liu
human	WB	Oncogene; Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.; Authors - Mengyi Lao ; KD Validated
		Am J Physiol Renal Physiol; TNF inhibits NKCC2 phosphorylation by a calcineurin-dependent pathway; Authors - Shoujin Hao
		Sci Rep; Calcineurin regulates cyclin D1 stability through dephosphorylation at T286.; Authors - Takahiro Goshima