PPP4R2 Polyklonaler Antikörper

PPP4R2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 22676-1-AP

Synonyme

PP4R2, PPP4R2

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  WB analysis of A549 using 22676-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22676-1-AP (PPP4R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22676-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22676-1-AP (PPP4R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 22676-1-AP

  IP result of anti-PPP4R2 (IP:22676-1-AP, 4ug; Detection:22676-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of human liver cancer using 22676-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 22676-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 22676-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 22676-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of HepG2 using 22676-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 22676-1-AP (PPP4R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

22676-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PPP4R2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP4R2 fusion protein Ag18486
• Vollständiger Name: protein phosphatase 4, regulatory subunit 2
• Berechnetes Molekulargewicht: 360aa,40 kDa; 495aa,55 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC110889
• Gene symbol: PPP4R2
• Gene ID (NCBI): 151987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP4R2 is a regulatory subunit of protein phosphatase-4 (PPP4), a protein serine/threonine phosphatase implicated in microtubule organization at centrosomes and DNA double-strand break repair. The deduced 453-amino acid protein contains several potential phosphorylation sites and has a calculated molecular mass of 50.4 kD. Immunofluorescent analysis of HeLa and A431 cells revealed prominent localization of PPP4R2 at centrosomes, with weaker staining of cytoplasm and nucleus(PMID: 10769191).

Publikationen

Species	Application	Title
human	WB,CoIP	Exp Cell Res; Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs; Authors - Zhengwei Liang