PPPDE1/PNAS4 Polyklonaler Antikörper

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• KD/KO Validated

PPPDE1/PNAS4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 20517-1-AP

Synonyme

PPPDE1, EC:3.1.2.22, Deubiquitinase DESI2, Desumoylating isopeptidase 2, DeSI-2

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  WB analysis of A549 cells using 20517-1-AP

  WB result of PPPDE1 antibody (20517-1-AP, 1:1000) with A549 cells transfected with si-control and si-PPPDE1.

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  WB analysis using 20517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20517-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20517-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 20517-1-AP

  IP result of anti-PPPDE1/PNAS4 (IP:20517-1-AP, 4ug; Detection:20517-1-AP 1:500) with A549 cells lysate 2800ug.

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  IHC staining of human lung cancer using 20517-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 20517-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20517-1-AP

  Immunofluorescent analysis of HepG2 cells, using PPPDE1 antibody 20517-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, NCI-H1299-Zellen, U-251-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

20517-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PPPDE1/PNAS4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPPDE1/PNAS4 fusion protein Ag14352
• Vollständiger Name: PPPDE peptidase domain containing 1
• Berechnetes Molekulargewicht: 194 aa, 21 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC004485
• Gene symbol: PPPDE1
• Gene ID (NCBI): 51029
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPPDE peptidase domain 1 (PPPDE1), also named as PNAS4, is a highly conserved gene ubiquitously expressed in various tissues. PPPDE1 has been identified as a novel pro-apoptotic gene activated during the early response to DNA damage which is thought to play a critical role in cellular function regarding the maintenance of genomic integrity. This antibody specifically recognized endogenous PPPDE1, which has been confirmed by siRNA test.

Publikationen

Species	Application	Title
human	WB	Apoptosis; PPPDE1 promotes hepatocellular carcinoma development by negatively regulate p53 and apoptosis.; Authors - Xingwang Xie
rat	IHC, IF, WB	Exp Cell Res; Upregulation of PPPDE1 contributes to anorectal malformations via the mitochondrial apoptosis pathway during hindgut development in rats.; Authors - Si Ying Li
human,mouse	WB,IHC,IF	Biochimie; Comprehensive analysis of expression profile reveals the ubiquitous distribution of PPPDE peptidase domain 1, a Golgi apparatus component, and its implications in clinical cancer.; Authors - He Yi Y
human	WB	Biochem Biophys Res Commun; PPPDE1 is a novel deubiquitinase belonging to a cysteine isopeptidase family.; Authors - Xingwang Xie
human	IHC,WB	Pathol Oncol Res; High Phosphorylation Status of AKT/mTOR Signal in DESI2-Reduced Pancreatic Ductal Adenocarcinoma.; Authors - Cong-Cong Shen
human	WB,IHC	Oncol Lett; Analysis of transcription profile to reveal altered signaling pathways following the overexpression of human desumoylating isopeptidase 2 in pancreatic cancer cells.; Authors - Yu-Yin Fu