PPT1 Polyklonaler Antikörper

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• KD/KO Validated

PPT1 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29653-1-PBS

Synonyme

CLN1, EC:3.1.2.22, INCL, Palmitoyl-protein hydrolase 1, Palmitoyl-protein thioesterase 1

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  WB analysis using 29653-1-AP (same clone as 29653-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29653-1-AP (PPT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 29653-1-AP (same clone as 29653-1-PBS)

  WB result of PPT1 antibody (29653-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells. This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 29653-1-AP (same clone as 29653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 29653-1-AP (same clone as 29653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 10x lens). Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 29653-1-AP (same clone as 29653-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PPT1 antibody (29653-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29653-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA PPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPT1 fusion protein Ag31240
• Vollständiger Name: palmitoyl-protein thioesterase 1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 32-34 kDa
• GenBank-Zugangsnummer: BC008426
• Gene symbol: PPT1
• Gene ID (NCBI): 5538
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).