PQBP1 Polyklonaler Antikörper

PQBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16264-1-AP

Synonyme

Npw38, Polyglutamine tract-binding protein 1, Polyglutamine-binding protein 1, PQBP-1

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  WB analysis using 16264-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 16264-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16264-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16264-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16264-1-AP

  IP result of anti-PQBP1 (IP:16264-1-AP, 4ug; Detection:16264-1-AP 1:500) with mouse brain tissue lysate 3200 ug.

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  IHC staining of human breast cancer using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human colon using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16264-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PQBP1 antibody (16264-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

16264-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PQBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PQBP1 fusion protein Ag9329
• Vollständiger Name: polyglutamine binding protein 1
• Berechnetes Molekulargewicht: 265 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC012358
• Gene symbol: PQBP1
• Gene ID (NCBI): 10084
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PQBP1 (Polyglutamine-binding protein 1) is also named as NPW38 and JM26. PQBP1 senses extrinsic tau 3R/4R proteins by direct interaction and triggers an innate immune response by activating a cyclic GMP-AMP synthase (cGAS)-Stimulator of interferon genes (STING) pathway (PMID: 34782623).  PQBP1 is abundantly expressed in the central nervous system during development. PQBP1 is a component of the precatalytic spliceosome (B complex) that is involved in splicing. PQBP1 was predominantly localized in the cell nucleus. PQBP1 is overexpressed in various cancer types including breast cancer, colon cancer, and glioblastoma (PMID: 38342602).

Publikationen

Species	Application	Title
human	WB,IP,IF	Mol Cell; PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.; Authors - Yuqian Shen
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
mouse,human	WB,IP,IF	Cell Rep; Dynamic regulation of alternative polyadenylation by PQBP1 during neurogenesis; Authors - Xian Liu ; KD Validated
human	WB	mBio; Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response.; Authors - Hongming Ma