PQBP1 Polyklonaler Antikörper

PQBP1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16264-1-AP

Synonyme

MRX55, MRXS3, MRXS8, PQBP 1, PQBP1, RENS1, SHS

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Galerie der Validierungsdaten

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  WB analysis using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 16264-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16264-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16264-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16264-1-AP

  IP result of anti-PQBP1 (IP:16264-1-AP, 4ug; Detection:16264-1-AP 1:500) with mouse brain tissue lysate 3200 ug.

• 

  IHC staining of human breast cancer using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human colon using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16264-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16264-1-AP (PQBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

16264-1-AP bindet in WB, IP, IHC, IF, ELISA PQBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PQBP1 fusion protein Ag9329
• Vollständiger Name: polyglutamine binding protein 1
• Berechnetes Molekulargewicht: 265 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC012358
• Gene symbol: PQBP1
• Gene ID (NCBI): 10084
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB, IP, IF	Mol Cell; PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.; Authors - Yuqian Shen
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	mBio; Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response.; Authors - Hongming Ma