PRAS40 Polyklonaler Antikörper

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• KD/KO Validated

PRAS40 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 21097-1-AP

Synonyme

AKT1S1, 40 kDa proline rich AKT substrate, 40 kDa proline-rich AKT substrate, Proline rich AKT1 substrate 1, Proline-rich AKT1 substrate 1

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  WB analysis of MCF-7 using 21097-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21097-1-AP (PRAS40 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21097-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21097-1-AP (PRAS40 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21097-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 21097-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 21097-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21097-1-AP (PRAS40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

21097-1-AP bindet in WB, IHC, IF/ICC, ELISA PRAS40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRAS40 fusion protein Ag15355
• Vollständiger Name: AKT1 substrate 1 (proline-rich)
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC051844
• Gene symbol: PRAS40
• Gene ID (NCBI): 84335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRAS40 (40 kDa proline-rich AKT substrate) is also named as AKT1S1 (Proline-rich AKT1 substrate 1). PRAS40 is a substrate of Akt and a component of the mammalian target of rapamycin complex 1 (mTORC1) (PMID: 28978182). Locating at the crossroad of the PI3K/Akt pathway and the mTOR pathway, PRAS40 is phosphorylated by growth factors or other stimuli, and regulates the activation of these signaling pathways in turn (PMID: 28978182). PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB) proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2) (PMID: 33504218).

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice; Authors - Xiaoxue Jiang
mouse	WB	J Neuroinflammation; Sexually dimorphic extracellular vesicle responses after chronic spinal cord injury are associated with neuroinflammation and neurodegeneration in the aged brain; Authors - Yun Li
human	WB	Front Oncol; The Molecular Landscape and Biological Alterations Induced by PRAS40-Knockout in Head and Neck Squamous Cell Carcinoma.; Authors - Gang Chen ; KO Validated
		Mol Cell Oncol; Silencing Glypican-1 enhances the antitumor effects of Pictilisib via downregulating PI3K/Akt/ERK signaling in chemo-resistant esophageal adenocarcinoma; Authors - Akshay Pratap