PRC1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PRC1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E1D5

Kat-Nr. : 67027-1-PBS

Synonyme

1E1D5, ASE1

---

• 

  WB analysis using 67027-1-Ig (same clone as 67027-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67027-1-Ig (PRC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67027-1-Ig (same clone as 67027-1-PBS)

  WB result of PRC1 antibody (67027-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells. This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRC1 antibody (67027-1-Ig, Clone: 1E1D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRC1 antibody (67027-1-Ig, Clone: 1E1D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67027-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PRC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRC1 fusion protein Ag5907
• Vollständiger Name: protein regulator of cytokinesis 1
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 66-72 kDa
• GenBank-Zugangsnummer: BC003138
• Gene symbol: PRC1
• Gene ID (NCBI): 9055
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.