PRC1 Rekombinanter Antikörper

PRC1 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241216H2

Kat-Nr. : 84041-2-PBS

Synonyme

241216H2, ASE1

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  WB analysis using 84041-2-RR (same clone as 84041-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84041-2-RR (PRC1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00970-1

  Cytometric bead array standard curve of MP00970-1, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-1-PBS. Standard: Ag8028. Range: 0.625-80 ng/mL.

• 

  Cytometric bead array standard curve of MP00970-2

  Cytometric bead array standard curve of MP00970-2, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-3-PBS. Standard:null. Range: null.

• 

  Cytometric bead array standard curve of MP00970-2

  Cytometric bead array standard curve of MP00970-2, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-3-PBS. Standard: Ag8028. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84041-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84041-2-RR against Human PRC1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84041-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA PRC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PRC1 fusion protein Ag8028
• Vollständiger Name: protein regulator of cytokinesis 1
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 66-72 kDa
• GenBank-Zugangsnummer: BC003138
• Gene symbol: PRC1
• Gene ID (NCBI): 9055
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively.