PRC1 Rekombinanter Antikörper

PRC1 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241216H2

Kat-Nr. : CL488-84041-2

Synonyme

241216H2, ASE1

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  IF Staining of HepG2 using CL488-84041-2

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PRC1 antibody (CL488-84041-2, Clone: 241216H2 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of HepG2 using CL488-84041-2

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PRC1 antibody (CL488-84041-2, Clone: 241216H2 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of HepG2 using CL488-84041-2

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PRC1 antibody (CL488-84041-2, Clone: 241216H2 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of HepG2 using CL488-84041-2

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PRC1 antibody (CL488-84041-2, Clone: 241216H2 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of HepG2 using CL488-84041-2

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PRC1 antibody (CL488-84041-2, Clone: 241216H2 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-84041-2 bindet in IF/ICC PRC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PRC1 fusion protein Ag8028
• Vollständiger Name: protein regulator of cytokinesis 1
• Berechnetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC003138
• Gene symbol: PRC1
• Gene ID (NCBI): 9055
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively.