PRC1 Monoklonaler Antikörper

PRC1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1E1D5

Kat-Nr. : CL594-67027

Synonyme

ASE1, PRC1

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL594-67027

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PRC1 antibody (CL594-67027, Clone: 1E1D5 ) at dilution of 1:100.

• 

  IF Staining of HeLa using CL594-67027

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PRC1 antibody (CL594-67027, Clone: 1E1D5 ) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67027 bindet in IF PRC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRC1 fusion protein Ag5907
• Vollständiger Name: protein regulator of cytokinesis 1
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 66-72 kDa
• GenBank-Zugangsnummer: BC003138
• Gene symbol: PRC1
• Gene ID (NCBI): 9055
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.