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PRDM15 Polyklonaler Antikörper

PRDM15 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25590-1-PBS

Synonyme

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  • Western Blot (WB) analysis of Jurkat cells using PRDM15 Polyclonal antibody (25590-1-AP)

    WB analysis of Jurkat cells using 25590-1-AP (same clone as 25590-1-PBS)

    WB result of PRDM15 (25590-1-AP, 1:500) with si-control and si-PRDM15 transfected Jurkat cells. This data was developed using the same antibody clone with 25590-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PRDM15 Polyclonal antibody (25590-1-AP)

    WB analysis of MCF-7 using 25590-1-AP (same clone as 25590-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25590-1-AP (PRDM15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25590-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using PRDM15 Polyclonal antibody (25590-1-AP)

    IP experiment of MCF-7 using 25590-1-AP (same clone as 25590-1-PBS)

    IP result of anti-PRDM15 (IP:25590-1-AP, 5ug; Detection:25590-1-AP 1:500) with MCF-7 cells lysate 2500ug. This data was developed using the same antibody clone with 25590-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25590-1-PBS bindet in WB, IP, Indirect ELISA PRDM15 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PRDM15 fusion protein Ag22296
Vollständiger Name PR domain containing 15
Berechnetes Molekulargewicht 1507 aa, 169 kDa
Beobachtetes Molekulargewicht 169 kDa
GenBank-ZugangsnummerBC067102
Gene symbol PRDM15
Gene ID (NCBI) 63977
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PR-domain containing proteins (PRDMs) have a common involvement in the modulation of gene activities. A PR-domain family member usually produces two products, called PR-plus and PR-minus, which differ by the presence or absence of the PR domain, respectively. The PR-plus product is underexpressed or disrupted in cancer cells, whereas the PR-minus product is present or overexpressed in cancer cells. This imbalance in the amount of the two products, which is a result of either genetic or epigenetic events, appears to be a determining factor of malignancy. PRDM15 (PR domain-containing protein 15), also known as PFM15 or ZNF298, is a 1507 amino acid protein belonging to the PRDM family. Localizing to the nucleus, PRDM15 contains sixteen C2H2-type zinc fingers and one SET domain. It is believed to participate in transcriptional regulation and may be involved in cell differentiation and tumorigenesis. PRDM15 exists several isoform and molecular weight of respective isoform is 169 kDa, 135kda, 62kDa.