PRDM16 Rekombinanter Antikörper

PRDM16 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240662D3

Kat-Nr. : 83872-1-PBS

Synonyme

MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16, EC:2.1.1.367

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  WB analysis using 83872-1-RR (same clone as 83872-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83872-1-RR (PRDM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 83872-1-RR (same clone as 83872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83872-1-RR (PRDM16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 83872-1-RR (same clone as 83872-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PRDM16 antibody (83872-1-RR, Clone: 240662D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83872-1-PBS bindet in WB, IHC, IF/ICC, ELISA PRDM16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: PR domain containing 16
• Berechnetes Molekulargewicht: 140 kDa
• Beobachtetes Molekulargewicht: ~140 kDa
• GenBank-Zugangsnummer: NM_022114
• Gene symbol: PRDM16
• Gene ID (NCBI): 63976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PR domain containing 16, also known as PRDM16, acts as a transcription coregulator that controls the development of brown adipocytes in brown adipose tissue. Previously, this coregulator was believed to be present only in brown adipose tissue, but more recent studies have shown that PRDM16 is highly expressed in subcutaneous white adipose tissue as well. The protein encoded by this gene is a zinc finger transcription factor. PRDM16 controls the cell fate between muscle and brown fat cells. Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation.