PRDM8 Polyklonaler Antikörper

PRDM8 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26010-1-AP

Synonyme

EC:2.1.1.-, PR domain containing 8, PR domain containing protein 8, PR domain-containing protein 8

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  WB analysis using 26010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26010-1-AP (PRDM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 26010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26010-1-AP (PRDM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26010-1-AP (PRDM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 26010-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using PRDM8 antibody (26010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, Maus-Retina-Gewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26010-1-AP bindet in WB, IF/ICC, ELISA PRDM8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRDM8 fusion protein Ag23373
• Vollständiger Name: PR domain containing 8
• Berechnetes Molekulargewicht: 689 aa, 72 kDa
• Beobachtetes Molekulargewicht: 35 kDa, 72 kDa
• GenBank-Zugangsnummer: BC071584
• Gene symbol: PRDM8
• Gene ID (NCBI): 56978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888).