PRDM8 Rekombinanter Antikörper

PRDM8 Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242203D8

Kat-Nr. : 84716-2-PBS

Synonyme

242203D8, EC:2.1.1.-, PR domain containing 8, PR domain containing protein 8, PR domain-containing protein 8

---

• 

  WB analysis using 84716-2-RR (same clone as 84716-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84716-2-RR (PRDM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01532-1

  Cytometric bead array standard curve of MP01532-1, PRDM8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84716-2-PBS. Detection antibody: 84716-3-PBS. Standard: Ag23373. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84716-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84716-2-RR against Human PRDM8 were performed. The affinity constant is 31.8 nM.

---

Geprüfte Anwendungen

Produktinformation

84716-2-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA PRDM8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PRDM8 fusion protein Ag23373
• Vollständiger Name: PR domain containing 8
• Berechnetes Molekulargewicht: 689 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC071584
• Gene symbol: PRDM8
• Gene ID (NCBI): 56978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888).