PRDM8 Rekombinanter Antikörper

PRDM8 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

242203D8

Kat-Nr. : 84716-2-RR

Synonyme

242203D8, EC:2.1.1.-, PR domain containing 8, PR domain containing protein 8, PR domain-containing protein 8

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  WB analysis using 84716-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84716-2-RR (PRDM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 84716-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 84716-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 84716-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 84716-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84716-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84716-2-RR against Human PRDM8 were performed. The affinity constant is 31.8 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maus-Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84716-2-RR bindet in WB, IHC, IF-P, ELISA PRDM8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PRDM8 fusion protein Ag23373
• Vollständiger Name: PR domain containing 8
• Berechnetes Molekulargewicht: 689 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC071584
• Gene symbol: PRDM8
• Gene ID (NCBI): 56978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888).