PRDX1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRDX1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(47)

Rezensionen (2)

Kat-Nr. : 15816-1-AP

Synonyme

EC:1.11.1.24, MSP23, Natural killer cell-enhancing factor A, NKEF-A, PAG

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• 

  WB analysis using 15816-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15816-1-AP (PRDX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 15816-1-AP

  U2OS (WT and PRDX1 KO) lysates prepared with RIPA buffer, 10 μg protein loaded. 15816-1-AP incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis of HEK-293 using 15816-1-AP

  WB result of PRDX1 antibody (15816-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX1 transfected HEK-293 cells.

• 

  IP experiment of HEK-293 using 15816-1-AP

  IP result of anti-PRDX1 (IP:15816-1-AP, 4ug; Detection:15816-1-AP 1:20000) with HEK-293 cells lysate 1800 ug.

• 

  IP experiment of mouse kidney using 15816-1-AP

  IP result of anti-PRDX1 (IP:15816-1-AP, 4ug; Detection:15816-1-AP 1:20000) with mouse kidney tissue lysate 3400 ug.

• 

  IHC staining of human breast cancer using 15816-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15816-1-AP (PRDX1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15816-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15816-1-AP (PRDX1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 15816-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15816-1-AP (PRDX1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15816-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRDX1 antibody (15816-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, Rattennierengewebe, U2OS-Zellen
• Erfolgreiche IP: HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 40 publications below
• IHC: See 8 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15816-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PRDX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRDX1 fusion protein Ag8602
• Vollständiger Name: peroxiredoxin 1
• Berechnetes Molekulargewicht: 199 aa, 22 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC007063
• Gene symbol: PRDX1
• Gene ID (NCBI): 5052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX1 (Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (PMID: 9497357). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.; Authors - Haohao Huang
mouse	WB	Redox Biol; PMID: 32604037; Authors - Anindya Roy Chowdhury
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
		Cell Chem Biol; Parvifoline AA Promotes Susceptibility of Hepatocarcinoma to Natural Killer Cell-Mediated Cytolysis by Targeting Peroxiredoxin.; Authors - Huifang Zhu
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H
rat	WB	Elife; Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control; Authors - Meiling Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guillermo (Verified Customer) (07-20-2022)

Very nice product.

• Applications: Western Blot

FH

Wei (Verified Customer) (03-12-2020)

PRDX1 is easily detected in mouse hearts. It has strong, and clear band.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart