PRDX1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PRDX1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2B2A2

Rezensionen (1)

Kat-Nr. : 66820-1-Ig

Synonyme

MSP23, NKEF A, NKEFA, PAG, PAGA, PAGB, peroxiredoxin 1, PRDX1, PRX1, PRXI, TDPX2, Thioredoxin peroxidase 2

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Galerie der Validierungsdaten

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  WB analysis using 66820-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66820-1-Ig

  WB result of PRDX1 antibody (66820-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX1 transfected HEK-293 cells.

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  WB analysis of U2OS using 66820-1-Ig

  U2OS (WT and PRDX1 KO) lysates prepared with RIPA buffer, 10 μg protein loaded. 66820-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis using 66820-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66820-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66820-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 66820-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PRDX1 antibody (66820-1-Ig, Clone: 2B2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, Caco-2-Zellen, HEK-293-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66820-1-Ig bindet in WB, IP, IHC, IF, CoIP, ELISA PRDX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRDX1 fusion protein Ag8821
• Vollständiger Name: peroxiredoxin 1
• Berechnetes Molekulargewicht: 199 aa, 22 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC007063
• Gene symbol: PRDX1
• Gene ID (NCBI): 5052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 ((PMID: 9497357)). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).

Publikationen

Species	Application	Title
human	WB, CoIP	J Virol; Peroxiredoxin 1 interacts with TBK1/IKKε and negatively regulates pseudorabies virus propagation by promoting innate immunity.; Authors - Lin Lv
mouse	WB	Antioxidants (Basel); Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice; Authors - Ling Zhu
rat	IP	Heliyon; EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion; Authors - Zi-Jie Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

PILAR (Verified Customer) (08-24-2023)

Excellent customer service and fast shipment. Waiting for the experiment results!

• Applications: Western Blot