Peroxiredoxin 2 Rekombinanter Antikörper

Peroxiredoxin 2 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251494D3

Kat-Nr. : 86599-1-PBS

Synonyme

PRDX2, EC:1.11.1.24, Natural killer cell-enhancing factor B, NKEFB, NKEF-B

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  WB analysis using 86599-1-RR (same clone as 86599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86599-1-RR (Peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86599-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02542-2

  The mean Peroxiredoxin 2 concentration was determined to be 75.74 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA sample test of MP02542-2

  Plasma of six individual healthy human donors was measured. The human Peroxiredoxin 2 concentration of detected samples was determined to be 6.52 ng/mL with a range of 5.21 - 7.71 ng/mL.

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  Sandwich ELISA standard curve of MP02542-2

  Sandwich ELISA standard curve of MP02542-2, Human Peroxiredoxin 2 Recombinant Matched Antibody Pair - PBS only. 86599-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0835. 86599-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

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  IF Staining of HepG2 using 86599-1-RR (same clone as 86599-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Peroxiredoxin 2 antibody (86599-1-RR, Clone: 251494D3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86599-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02542-1

  The mean Peroxiredoxin 2 concentration was determined to be 144.8 ng/mL in human serum.

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  Cytometric bead array sample test of MP02542-1

  The mean Peroxiredoxin 2 concentration was determined to be 170.3 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02542-1

  Cytometric bead array standard curve of MP02542-1, Peroxiredoxin 2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86599-2-PBS. Detection antibody: 86599-1-PBS. Standard: Ag0835. Range: 3.125-100 ng/mL.

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  Affinity and Kinetic Characterization of 86599-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86599-1-RR against Human Peroxiredoxin 2 were performed. The affinity constant is 3.01 nM.

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Geprüfte Anwendungen

Produktinformation

86599-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA Peroxiredoxin 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peroxiredoxin 2 fusion protein Ag0835
• Vollständiger Name: peroxiredoxin 2
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC003022
• Gene symbol: peroxiredoxin 2
• Gene ID (NCBI): 7001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxiredoxin 2 (PRDX2), also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)