PRDX3 Polyklonaler Antikörper

PRDX3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 55087-1-AP

Synonyme

peroxiredoxin 3, MER5, EC:1.11.1.24, AOP-1, AOP1

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  WB analysis using 55087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55087-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55087-1-AP (PRDX3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 55087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PRDX3 antibody (55087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen, Mauslebergewebe, Rattenlebergewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

55087-1-AP bindet in WB, IHC, IF/ICC, ELISA PRDX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: peroxiredoxin 3
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 26-28 kDa
• GenBank-Zugangsnummer: NM_006793
• Gene symbol: PRDX3
• Gene ID (NCBI): 10935
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells(PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. This antibody is specific to PRDX3.

Publikationen

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
	WB	J Endocrinol; NNT is a key regulator of adrenal redox homeostasis and steroidogenesis in male mice.; Authors - Eirini Meimaridou
human	WB	J Cell Biochem; Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.; Authors - Sulpizio Marilisa M
rat	WB	Cardiovasc Toxicol; Nrf1 Knock-Down in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Intervention of Superoxide Production-Removal Balance and Mitochondrial Function.; Authors - Ying Li
mouse	WB,IF	Respir Res; Peroxiredoxin 3 has a crucial role in the macrophage polarization by regulating mitochondrial homeostasis; Authors - Wenhui Huang