PRDX3 Monoklonaler Antikörper

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• KD/KO Validated

PRDX3 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F7F7

Kat-Nr. : 66810-1-PBS

Synonyme

1F7F7, Antioxidant protein 1, AOP1, AOP-1, EC:1.11.1.24

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  WB analysis using 66810-1-Ig (same clone as 66810-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRX3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66810-1-Ig (same clone as 66810-1-PBS)

  WB result of PRDX3 antibody (66810-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  WB analysis using 66810-1-Ig (same clone as 66810-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRDX3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66810-1-Ig (same clone as 66810-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66810-1-Ig (PRX3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66810-1-Ig (same clone as 66810-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66810-1-Ig (PRX3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66810-1-Ig (same clone as 66810-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (66810-1-Ig, Clone: 1F7F7 ) at dilution of 1:1700 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50218-2

  Cytometric bead array standard curve of MP50218-2, PRDX3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66810-1-PBS. Detection antibody: 66810-2-PBS. Standard: Ag1062. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 66810-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66810-1-Ig against Human PRDX3 were performed. The affinity constant is 1.39 nM.

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Geprüfte Anwendungen

Produktinformation

66810-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA PRDX3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRDX3 fusion protein Ag1062
• Vollständiger Name: peroxiredoxin 3
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC007062
• Gene symbol: PRDX3
• Gene ID (NCBI): 10935
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.