PRDX4 Polyklonaler Antikörper

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• KD/KO Validated

PRDX4 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Kat-Nr. : 10703-1-AP

Synonyme

Peroxiredoxin IV, peroxiredoxin 4, EC:1.11.1.24, AOE37-2, AOE37 2

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  WB analysis using 10703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10703-1-AP (PRDX4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10703-1-AP

  WB result of PRDX4 antibody (10703-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX4 transfected HepG2 cells.

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  IP experiment of HepG2 using 10703-1-AP

  IP result of anti-PRDX4 (IP:10703-1-AP, 4ug; Detection:10703-1-AP 1:4000) with HepG2 cells lysate 2040 ug.

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  IHC staining of human colon cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10703-1-AP (PRDX4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10703-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRDX4 antibody (10703-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10703-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PRDX4 (10703-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

10703-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA PRDX4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRDX4 fusion protein Ag1177
• Vollständiger Name: peroxiredoxin 4
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC007107
• Gene symbol: PRDX4
• Gene ID (NCBI): 10549
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.

Publikationen

Species	Application	Title
human,mouse	WB	Nat Commun; Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice; Authors - Wen Yin
human	WB,IP	Nat Commun; The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma; Authors - Alexander Vdovin
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
human	WB	Antioxid Redox Signal; Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I.; Authors - Xiao-Jing Shi
human	WB	Antioxid Redox Signal; Direct Proteomic Mapping of Cysteine Persulfidation.; Authors - Ling Fu
mouse	WB	Bioorg Chem; Novel [1,2,3]triazolo[4,5-d]pyrimidine derivatives containing hydrazone fragment as potent and selective anticancer agents.; Authors - Chenhao Xu