PRDX4 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PRDX4 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

5B6F1

Kat-Nr. : 60286-1-Ig

Synonyme

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 60286-1-Ig

  WB result of PRDX4 antibody (60286-1-Ig, 1:5000) with si-Control and si-PRDX4 transfected HepG2 cells.

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  WB analysis using 60286-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 60286-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 60286-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60286-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60286-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 60286-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(unde10x lens).

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  IHC staining of human colon using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of human pancreas cancer using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human pancreas cancer using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of human prostate cancer using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human prostate cancer using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens).

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  IF Staining of HepG2 using 60286-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60286-1-Ig (PRDX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Pankreaskarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

60286-1-Ig bindet in WB, IHC, IF, ELISA PRDX4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRDX4 fusion protein Ag13401
• Vollständiger Name: peroxiredoxin 4
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC007107
• Gene symbol: PRDX4
• Gene ID (NCBI): 10549
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).

Publikationen

Species	Application	Title
human	WB	Nat Commun; The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma; Authors - Alexander Vdovin ; KD Validated
mouse	IF	Biochem Biophys Res Commun; Peroxiredoxin 4 secreted by cumulus cells ameliorates the maturation of oocytes in vitro; Authors - Wangjuan Dai