PRX5 Polyklonaler Antikörper

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• KD/KO Validated

PRX5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 17724-1-AP

Synonyme

ACR1, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, AOPP, B166, Liver tissue 2D page spot 71B, Peroxiredoxin 5, Peroxiredoxin 5, mitochondrial, Peroxiredoxin V, Peroxisomal antioxidant enzyme, PLP, PMP20, PRDX5, Prx V, PRX5, PRXV, SBBI10, Thioredoxin peroxidase PMP20, Thioredoxin reductase, TPx type VI

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Galerie der Validierungsdaten

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  WB analysis using 17724-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17724-1-AP (PRX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17724-1-AP

  WB result of PRX5 antibody (17724-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRX5 transfected HepG2 cells.

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  IHC staining of human kidney using 17724-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17724-1-AP (PRX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17724-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17724-1-AP (PRX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HepG2-Zellen, Mausnierengewebe, Rattennierengewebe, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IF: See 3 publications below

Produktinformation

17724-1-AP bindet in WB, IHC, IF, ELISA PRX5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRX5 fusion protein Ag12118
• Vollständiger Name: peroxiredoxin 5
• Berechnetes Molekulargewicht: 214 aa, 22 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC110983
• Gene symbol: PRDX5
• Gene ID (NCBI): 25824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)

Publikationen

Species	Application	Title
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
human,mouse	WB,IF	FASEB J; Antioxidant enzyme peroxiredoxin 5 regulates cyst growth and ciliogenesis via modulating Plk1 stability.; Authors - Ewud Agborbesong ; KD Validated
mouse	WB	Bioorg Chem; Novel [1,2,3]triazolo[4,5-d]pyrimidine derivatives containing hydrazone fragment as potent and selective anticancer agents.; Authors - Chenhao Xu
mouse	WB	J Cell Mol Med; PMID: 21251210; Authors - Xi Lei L
mouse	WB	J Biol Chem; mTOR Inhibition with Rapamycin Improves Cardiac Function in Type 2 Diabetic Mice: Potential Role of Attenuated Oxidative Stress and Altered Contractile Protein Expression.; Authors - Anindita Das
human	WB	Sci Rep; LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells.; Authors - Wenchuan Qi