PRDX6 Rekombinanter Antikörper

PRDX6 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

251053E8

Kat-Nr. : 86368-1-PBS

Synonyme

1-Cys peroxiredoxin, 1-Cys PRX, Acidic calcium-independent phospholipase A2, AOP2, EC:1.11.1.27

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  WB analysis using 86368-1-RR (same clone as 86368-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86368-1-RR (PRDX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86368-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02551-1

  Sandwich ELISA standard curve of MP02551-1, Human PRDX6 Recombinant Matched Antibody Pair - PBS only. 86368-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4512. 86368-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02551-1

  Plasma of six individual healthy human donors was measured. The human PRDX6 concentration of detected samples was determined to be 0.80 ng/mL with a range of 0.27 - 1.25 ng/mL.

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  Affinity and Kinetic Characterization of 86368-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86368-1-RR against Human PRDX6 were performed. The affinity constant is 0.234 nM.

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Geprüfte Anwendungen

Produktinformation

86368-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test PRDX6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PRDX6 fusion protein Ag4512
• Vollständiger Name: peroxiredoxin 6
• Berechnetes Molekulargewicht: 224 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC035857
• Gene symbol: PRDX6
• Gene ID (NCBI): 9588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).