PREB Monoklonaler Antikörper

PREB Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F6A1

Kat-Nr. : 66981-1-PBS

Synonyme

2F6A1, Guanine nucleotide-exchange factor SEC12, Prolactin regulatory element-binding protein, SEC12

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  WB analysis of Daudi using 66981-1-Ig (same clone as 66981-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66981-1-Ig (same clone as 66981-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66981-1-Ig (same clone as 66981-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66981-1-Ig (same clone as 66981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66981-1-Ig (same clone as 66981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66981-1-PBS bindet in WB, IHC, Indirect ELISA PREB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PREB fusion protein Ag28549
• Vollständiger Name: prolactin regulatory element binding
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC002765
• Gene symbol: PREB
• Gene ID (NCBI): 10113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.