PRELP Polyklonaler Antikörper

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• KD/KO Validated

PRELP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 23783-1-AP

Synonyme

SLRR2A, Proline-arginine-rich end leucine-rich repeat protein, Prolargin, MSTP161, MST161

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  WB analysis using 23783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23783-1-AP (PRELP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 23783-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23783-1-AP (PRELP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 23783-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23783-1-AP (PRELP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 23783-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PRELP antibody (23783-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

23783-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA PRELP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRELP fusion protein Ag20656
• Vollständiger Name: proline/arginine-rich end leucine-rich repeat protein
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC032498
• Gene symbol: PRELP
• Gene ID (NCBI): 5549
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRELP (proline/arginine-rich end leucine-rich repeat protein), also known as MST161, MSTP161, prolargin, or SLRR2A, belongs to the class II subfamily of the small leucine-rich proteoglycan (SLRP) family. PRELP is a small leucine-rich proteoglycan (SLRP), which binds collagen to basement membranes or cartilage as a matrix protein. PRELP has prognostic value and regulates cell proliferation and migration in hepatocellular carcinoma, may serve as a potential prognostic marker and a treatment target against HCC progression (PMID: 33033521).

Publikationen

Species	Application	Title
human	WB,IF,CoIP	Apoptosis; PRELP inhibits colorectal cancer progression by suppressing epithelial-mesenchymal transition and angiogenesis via the inactivation of the FGF1/PI3K/AKT pathway; Authors - Xiaoqing Li ; KD Validated
human	WB,IHC	Front Med (Lausanne); Investigating the role of disulfidptosis related genes in radiotherapy resistance of lung adenocarcinoma; Authors - Xiaoxia Pan