PREP Polyklonaler Antikörper

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• KD/KO Validated

PREP Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11536-1-PBS

Synonyme

Post-proline cleaving enzyme, PEP, EC:3.4.21.26

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  WB analysis of human lung using 11536-1-AP (same clone as 11536-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 11536-1-AP (same clone as 11536-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11536-1-AP (same clone as 11536-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11536-1-AP (same clone as 11536-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11536-1-AP (PREP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 11536-1-AP (same clone as 11536-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 11536-1-AP (PREP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11536-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PREP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PREP fusion protein Ag2101
• Vollständiger Name: prolyl endopeptidase
• Berechnetes Molekulargewicht: 710 aa, 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC030636
• Gene symbol: PREP
• Gene ID (NCBI): 5550
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)