PREPL Polyklonaler Antikörper

PREPL Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12478-1-PBS

Synonyme

EC:3.4.21.-, KIAA0436, PPCEL, prolyl endopeptidase like, Prolyl endopeptidase-like

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  WB analysis of human brain using 12478-1-AP (same clone as 12478-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12478-1-AP (same clone as 12478-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12478-1-AP (same clone as 12478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12478-1-AP (same clone as 12478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12478-1-AP (same clone as 12478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12478-1-AP (same clone as 12478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12478-1-PBS bindet in WB, IHC, Indirect ELISA PREPL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PREPL fusion protein Ag3196
• Vollständiger Name: prolyl endopeptidase-like
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC013193
• Gene symbol: PREPL
• Gene ID (NCBI): 9581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PREPL (Prolyl endopeptidase-like) is also named as KIAA0436 and belongs to the prolyl oligopeptidase subfamily of serine peptidases. IPREPL is a novel oligopeptidase with unique structural and functional characteristics (PMID:16385448). It has 4 isoforms produced by alternative splicing and can exsit as a homodimer (PMID:16143824). Defects in PREPL are a cause of hypotonia-cystinuria syndrome (HCS).