PREX1 Polyklonaler Antikörper

PREX1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33193-1-AP

Synonyme

KIAA1415, P REX1, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein, P-Rex1, PtdIns(3,4,5)-dependent Rac exchanger 1

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  WB analysis using 33193-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33193-1-AP (PREX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 33193-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PREX1 antibody (33193-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

33193-1-AP bindet in WB, IF/ICC, ELISA PREX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PREX1 fusion protein Ag36649
• Vollständiger Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
• Berechnetes Molekulargewicht: 186 kDa
• Beobachtetes Molekulargewicht: 186 kDa
• GenBank-Zugangsnummer: NM_020820.3
• Gene symbol: PREX1
• Gene ID (NCBI): 57580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (PREX1) is a guanine nucleotide exchange factor (GEF) that specifically activates Rac GTPases, particularly Rac1 (PMID: 16243035). PREX1 is activated downstream of G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), such as ErbB receptors, and is involved in PI3K/AKT and MAPK signaling pathways (PMID: 28710285; 27358402). It contributes to glucose uptake in adipocytes and promotes tumor cell proliferation, migration, and metastasis in various cancers, including breast, prostate, and melanoma (PMID: 22109529).