PRICKLE1 Rekombinanter Antikörper

PRICKLE1 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242506A8

Kat-Nr. : 84926-1-PBS

Synonyme

242506A8, EPM1B, Prickle-like protein 1, REST/NRSF-interacting LIM domain protein 1, RILP

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  WB analysis of MDA-MB-231 using 84926-1-RR (same clone as 84926-1-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 84926-1-RR (PRICKLE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84926-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 84926-1-RR (same clone as 84926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84926-1-RR (PRICKLE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84926-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01670-2

  Cytometric bead array standard curve of MP01670-2, PRICKLE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84926-3-PBS. Detection antibody: 84926-1-PBS. Standard: Ag18407. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84926-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84926-1-RR against Human PRICKLE1 were performed. The affinity constant is 7.50 nM.

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Geprüfte Anwendungen

Produktinformation

84926-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA PRICKLE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PRICKLE1 fusion protein Ag18407
• Vollständiger Name: prickle homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 831 aa, 94 kDa
• Beobachtetes Molekulargewicht: 94 kDa
• GenBank-Zugangsnummer: BC114939
• Gene symbol: PRICKLE1
• Gene ID (NCBI): 144165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).