PRIM1 Polyklonaler Antikörper

PRIM1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10773-1-PBS

Synonyme

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  WB analysis using 10773-1-AP (same clone as 10773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10773-1-AP (same clone as 10773-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Sp2/0 using 10773-1-AP (same clone as 10773-1-PBS)

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 10773-1-AP (same clone as 10773-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Sp2/0 using 10773-1-AP (same clone as 10773-1-PBS)

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 10773-1-AP (same clone as 10773-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10773-1-AP (same clone as 10773-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 10773-1-AP (same clone as 10773-1-PBS)

  IP result of anti-PRIM1 (IP:10773-1-AP, 4ug; Detection:10773-1-AP 1:300) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 10773-1-AP (same clone as 10773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10773-1-AP (PRIM1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10773-1-AP (same clone as 10773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10773-1-AP (PRIM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10773-1-AP (same clone as 10773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10773-1-AP (PRIM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10773-1-AP (same clone as 10773-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRIM1 antibody (10773-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10773-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PRIM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRIM1 fusion protein Ag1124
• Vollständiger Name: primase, DNA, polypeptide 1 (49kDa)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC005266
• Gene symbol: PRIM1
• Gene ID (NCBI): 5557
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA replication in human cells is initiated by a complex apparatus containing a DNA polymerase-alpha/primase complex. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. PRIM1 is a subnuit of this complex.