AMPK Alpha Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AMPK Alpha Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(300)

Rezensionen (9)

Kat-Nr. : 10929-2-AP

Synonyme

AMPK Alpha 1, PRKAA1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AAPK1, ACACA kinase

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  WB analysis using 10929-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10929-2-AP (AMPK Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10929-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10929-2-AP (AMPK Alpha 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10929-2-AP

  IP result of anti-AMPK Alpha (IP:10929-2-AP, 4ug; Detection:10929-2-AP 1:1000) with HeLa cells lysate 1600 ug.

• 

  IHC staining of human prostate cancer using 10929-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10929-2-AP (AMPK Alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10929-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10929-2-AP (AMPK Alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10929-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMPK Alpha antibody (10929-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 285 publications below
• IHC: See 7 publications below
• IF: See 10 publications below
• IP: See 3 publications below

Produktinformation

10929-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA AMPK Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Fisch, Hausschwein, Kaninchen, Maus, Ratte, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMPK Alpha fusion protein Ag1367
• Vollständiger Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC012622
• Gene symbol: AMPK Alpha 1
• Gene ID (NCBI): 5562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.10929-2-AP can recognize both AMPK Alpha 1 and AMPK Alpha 2.

Publikationen

Species	Application	Title
mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
		Signal Transduct Target Ther; Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification; Authors - Zhifan Mao
mouse	WB	Adv Sci (Weinh); Mitigating Doxorubicin-Induced Cardiotoxicity and Enhancing Anti-Tumor Efficacy with a Metformin-Integrated Self-Assembled Nanomedicine; Authors - Jiaxin Huang
human	WB	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	WB,IP	Autophagy; The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses.; Authors - Yongquan Hu ; KO Validated
rat,human	WB	J Adv Res; A dual absorption pathway of novel oyster-derived peptide-zinc complex enhances zinc bioavailability and restores mitochondrial function; Authors - Ximing Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nishita (Verified Customer) (09-12-2025)

Worked pretty well, but we had to use a more concentrated dilution than the one recommended by the site.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: NB1 Fibroblasts

FH

Priya (Verified Customer) (10-03-2024)

Used this antibody for colon carcinoma cell line and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Charlotte (Verified Customer) (08-15-2024)

Done in milk but got quite some background. I tried in seablock but not sure it helps. Otherwise nice antibody with one intense band at the expected MW

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: NIH-3T3

AMPK Alpha Antibody Western Blot validation (1/5000 dilution) in NIH-3T3 (Cat no:10929-2-AP)

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jing (Verified Customer) (01-27-2020)

tested in mouse heart lysate, with clear single band around 60 kd.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart lysate

FH

Jie (Verified Customer) (01-27-2020)

showed a single band at the correct size with mouse heart tissue section

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse heart tissue

FH

Yan (Verified Customer) (01-03-2020)

We tested this antibody in mouse heart tissue extract. It worked very well and showed a single band around 60kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue