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AMPK Alpha Polyklonaler Antikörper
AMPK Alpha Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (5)
Anwendung
WB, IHC, IF/ICC, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10929-2-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen |
| Erfolgreiche IP | HeLa-Zellen |
| Erfolgreiche Detektion in IHC | humanes Prostatakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 12 publications below |
| WB | See 285 publications below |
| IHC | See 7 publications below |
| IF | See 10 publications below |
| IP | See 3 publications below |
Produktinformation
10929-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA AMPK Alpha und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Fisch, Hausschwein, Kaninchen, Maus, Ratte, Zebrafisch, Ziege |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | AMPK Alpha fusion protein Ag1367 |
| Vollständiger Name | protein kinase, AMP-activated, alpha 1 catalytic subunit |
| Berechnetes Molekulargewicht | 63 kDa |
| Beobachtetes Molekulargewicht | 64 kDa |
| GenBank-Zugangsnummer | BC012622 |
| Gene symbol | AMPK Alpha 1 |
| Gene ID (NCBI) | 5562 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.10929-2-AP can recognize both AMPK Alpha 1 and AMPK Alpha 2.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for AMPK Alpha antibody 10929-2-AP | Protokoll herunterladen |
| IHC protocol for AMPK Alpha antibody 10929-2-AP | Protokoll herunterladenl |
| IF protocol for AMPK Alpha antibody 10929-2-AP | Protokoll herunterladen |
| IP protocol for AMPK Alpha antibody 10929-2-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification | ||
Adv Sci (Weinh) Mitigating Doxorubicin-Induced Cardiotoxicity and Enhancing Anti-Tumor Efficacy with a Metformin-Integrated Self-Assembled Nanomedicine | ||
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Autophagy The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses.
| ||
J Adv Res A dual absorption pathway of novel oyster-derived peptide-zinc complex enhances zinc bioavailability and restores mitochondrial function |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Nishita (Verified Customer) (09-12-2025) | Worked pretty well, but we had to use a more concentrated dilution than the one recommended by the site.
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FH Priya (Verified Customer) (10-03-2024) | Used this antibody for colon carcinoma cell line and mice tissue
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FH Charlotte (Verified Customer) (08-15-2024) | Done in milk but got quite some background. I tried in seablock but not sure it helps. Otherwise nice antibody with one intense band at the expected MW
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FH Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells andmice tissue
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FH Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells andmice tissue
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FH LUNFENG (Verified Customer) (01-27-2020) | GOOD
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FH Jing (Verified Customer) (01-27-2020) | tested in mouse heart lysate, with clear single band around 60 kd.
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FH Jie (Verified Customer) (01-27-2020) | showed a single band at the correct size with mouse heart tissue section
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FH Yan (Verified Customer) (01-03-2020) | We tested this antibody in mouse heart tissue extract. It worked very well and showed a single band around 60kDa.
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