AMPK Beta 2 Polyklonaler Antikörper

AMPK Beta 2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 14429-1-AP

Synonyme

PRKAB2, 5'-AMP-activated protein kinase subunit beta-2, AMPK, AMPK subunit beta 2, AMPK subunit beta-2

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  WB analysis using 14429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14429-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14429-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 14429-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 14429-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14429-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14429-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14429-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14429-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (14429-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, fetales humanes Hirngewebe, humanes Hirngewebe, humanes Herzgewebe, Maushirngewebe, Maushodengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14429-1-AP bindet in WB, IHC, IF/ICC, ELISA AMPK Beta 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMPK Beta 2 fusion protein Ag5806
• Vollständiger Name: protein kinase, AMP-activated, beta 2 non-catalytic subunit
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC053610
• Gene symbol: AMPK Beta 2
• Gene ID (NCBI): 5565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

YINGJIAN (Verified Customer) (08-15-2025)

high specificity, strong/robust signal, low background, consistent results, reproducible performance, good lot-to-lot consistency

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: heart

AMPK Beta 2 Antibody Western Blot validation (1:5000 dilution) in heart (Cat no:14429-1-AP)