PRKACA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRKACA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 27398-1-AP

Synonyme

PKACA, PKA C-alpha, PKA C alpha, EC:2.7.11.11, cAMP-dependent protein kinase catalytic subunit alpha

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  WB analysis using 27398-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27398-1-AP (PRKACA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27398-1-AP

  WB result of PRKACA antibody (27398-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells.

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  IP experiment of HeLa using 27398-1-AP

  IP result of anti-PRKACA (IP:27398-1-AP, 4ug; Detection:27398-1-AP 1:300) with HeLa cells lysate 2600 ug.

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  IHC staining of human testis using 27398-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 27398-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 27398-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 27398-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of U2OS using 27398-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PRKACA antibody (27398-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Neuro-2a-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IP: See 1 publications below

Produktinformation

27398-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PRKACA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKACA fusion protein Ag26392
• Vollständiger Name: protein kinase, cAMP-dependent, catalytic, alpha
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 38-43 kDa
• GenBank-Zugangsnummer: BC039846
• Gene symbol: PRKACA
• Gene ID (NCBI): 5566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Front Pharmacol; Phosphoproteomic and proteomic profiling in post-infarction chronic heart failure; Authors - Jiayue Wang
chicken	WB	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
human,mouse	WB,IP	Eur J Pharmacol; Theaflavine inhibits hepatic stellate cell activation by modulating the PKA/LKB1/AMPK/GSK3β cascade and subsequently enhancing Nrf2 signaling; Authors - Guangwen Shu
human	WB	Mediators Inflamm; Vitronectin Destroyed Intestinal Epithelial Cell Differentiation through Activation of PDE4-Mediated Ferroptosis in Inflammatory Bowel Disease; Authors - Wenxu Pan
human	WB	Cell Rep; Protein modifications throughout the lung cancer proteome unravel the cancer-specific regulation of glycolysis.; Authors - Yangmiao Duan ; KD Validated
mouse	WB	Physiol Behav; Protection of p-Coumaric acid against chronic stress-induced neurobehavioral deficits in mice via activating the PKA-CREB-BDNF pathway; Authors - Yanqun Cao