PRKACB Polyklonaler Antikörper

PRKACB Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12232-1-AP

Synonyme

PKA C beta, PKACB, PRKACB

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Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 12232-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12232-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12232-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12232-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12232-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12232-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12232-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12232-1-AP

  IP Result of anti-PRKACB (IP:12232-1-AP, 4ug; Detection:12232-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of mouse brain using 12232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human medulloblastoma using 12232-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12232-1-AP (PRKACB antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 12232-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKACB antibody (12232-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, HT-1080.Zellen, Mausherzgewebe, Maushirngewebe, PC-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Medulloblastom-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12232-1-AP bindet in WB, IP, IHC, IF, ELISA PRKACB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKACB fusion protein Ag2870
• Vollständiger Name: protein kinase, cAMP-dependent, catalytic, beta
• Berechnetes Molekulargewicht: 41 kDa, 46 kDa
• Beobachtetes Molekulargewicht: 36-55 kDa
• GenBank-Zugangsnummer: BC016285
• Gene symbol: PRKACB
• Gene ID (NCBI): 5567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.

Publikationen

Species	Application	Title
mouse	WB	Int J Nanomedicine; Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway.; Authors - Yang Fu
mouse,rat	WB	Biomed Pharmacother; Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.; Authors - Yang Fu
human	WB	Biomed Res Int; MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB.; Authors - Yongxia Wang
human	WB	Int J Mol Sci; Dynamic Alteration Profile and New Role of RNA m6A Methylation in Replicative and H2O2-Induced Premature Senescence of Human Embryonic Lung Fibroblasts; Authors - Fan Wu
human	IHC	Front Med (Lausanne); A glycometabolic gene signature associating with immune infiltration and chemosensitivity and predicting the prognosis of patients with osteosarcoma; Authors - Fengyan Wang
mouse	WB,IF,IP	CNS Neurosci Ther; Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation; Authors - Limin Ma