AMPK Gamma 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AMPK Gamma 1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10290-1-AP

Synonyme

AMPK Gamma 1, AMPK gamma1, AMPK subunit gamma 1, AMPKG, PRKAG1

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Galerie der Validierungsdaten

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  WB analysis using 10290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10290-1-AP (AMPK Gamma 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10290-1-AP

  WB result of AMPK Gamma 1 antibody (10290-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK Gamma 1 transfected HEK-293 cells.

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  IP experiment of K-562 using 10290-1-AP

  IP result of anti-AMPK Gamma 1(IP:10290-1-AP, 4ug; Detection:10290-1-AP 1:1000) with K-562 cells lysate 3680 ug.

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  IHC staining of human testis using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10290-1-AP (AMPK gamma 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below

Produktinformation

10290-1-AP bindet in WB, IP, IHC, IF, ELISA AMPK Gamma 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMPK Gamma 1 fusion protein Ag0302
• Vollständiger Name: protein kinase, AMP-activated, gamma 1 non-catalytic subunit
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC000358
• Gene symbol: PRKAG1
• Gene ID (NCBI): 5571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1, synonyms: AMPKG, MGC8666) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an catalytic subunit, and non-catalytic and subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and iAMP-activated protein kinase (AMPK) is a highly conserved heterotrimeric serine/threonine kinase widely characterised as a sensor of cellular energetic stress. AMPK is a heterotrimeric complex consisting of a catalytic α-subunit and two regulatory subunits (β and γ). AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma 1 is one of the gamma regulatory subunits of AMPK.

Publikationen

Species	Application	Title
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
mouse	WB	JHEP Rep; Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis; Authors - Fuji Yang
human	WB	J Med Chem; Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.; Authors - Yuwen Sheng
human	WB	Protein Cell; AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth.; Authors - Zhu Kun K
human	WB	J Exp Clin Cancer Res; METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progression; Authors - Wei Wei ; KD Validated
human	WB	Cell Death Dis; WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.; Authors - Zhe Zheng