AMPK Gamma 2 Polyklonaler Antikörper

AMPK Gamma 2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12568-1-AP

Synonyme

AMPK gamma2, PRKAG2, 5'-AMP-activated protein kinase subunit gamma-2, AAKG, AAKG2

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  WB analysis using 12568-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12568-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12568-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 12568-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12568-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12568-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maus-Augengewebe, Neuro-2a-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

12568-1-AP bindet in WB, IHC, IF, ELISA AMPK Gamma 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMPK Gamma 2 fusion protein Ag3214
• Vollständiger Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• Berechnetes Molekulargewicht: 569 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa, 38 kDa
• GenBank-Zugangsnummer: BC020540
• Gene symbol: PRKAG2
• Gene ID (NCBI): 51422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.

Publikationen

Species	Application	Title
human	WB	J Med Chem; Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.; Authors - Yuwen Sheng
human	WB,IF	J Cardiol; Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway.; Authors - Zhang Bi-Li BL