PRKAR1A Polyklonaler Antikörper

PRKAR1A Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20358-1-PBS

Synonyme

PPNAD1, PKR1, CNC1, CNC, CAR

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  WB analysis using 20358-1-AP (same clone as 20358-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of rat salivary gland using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of rat salivary gland using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20358-1-AP (PRKAR1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKAR1A antibody (20358-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20358-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PRKAR1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKAR1A fusion protein Ag14216
• Vollständiger Name: protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
• Berechnetes Molekulargewicht: 381 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC036285
• Gene symbol: PRKAR1A
• Gene ID (NCBI): 5573
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKA is a heterotetramer inactive kinase composed of two regulatory and two catalytic submits. The regulatory subunits are encoded by four genes (PRKAR1A, PRKAR2A, PRKAR1B, and PRKAR2B). PRKAR1A, a gene coding for the cAMP-dependent protein kinase (PKA) 1α regulatory submit, is located on human chromosome 17q22-24. PRKAR1A protein insufficiency and PKA dysregulation have been implicated in various types of disorders, including Albright hereditary osteodystrophy (AHO), pseudohypoparathyroidism (PHP), acrodysostosis (ACRDYS), and Carney complex. PRKAR1A protein expression level was significantly dysregulated in multiple primary carcinomas and distant metastases, such as cardiac myxomas, odontogenic myxomas, anaplastic thyroid carcinomas, breast cancer, pediatric pituitary adenomas, and Schwann cell tumors.