PRKAR2A Monoklonaler Antikörper

PRKAR2A Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B2B3

Kat-Nr. : 67751-1-PBS

Synonyme

PKR2, PRKAR2, PRKAR2A

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  WB analysis using 67751-1-Ig (same clone as 67751-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67751-1-Ig (PRKAR2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRKAR2A antibody (67751-1-Ig, Clone: 1B2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67751-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PRKAR2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRKAR2A fusion protein Ag29893
• Vollständiger Name: protein kinase, cAMP-dependent, regulatory, type II, alpha
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002763
• Gene symbol: PRKAR2A
• Gene ID (NCBI): 5576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.