PRKAR2B Polyklonaler Antikörper

PRKAR2B Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28351-1-PBS

Synonyme

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

---

• 

  WB analysis using 28351-1-AP (same clone as 28351-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28351-1-AP (same clone as 28351-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 28351-1-AP (same clone as 28351-1-PBS)

  IP result of anti-PRKAR2B (IP:28351-1-AP, 4ug; Detection:28351-1-AP 1:1000) with mouse brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 28351-1-AP (same clone as 28351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 28351-1-AP (same clone as 28351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 28351-1-AP (same clone as 28351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 28351-1-AP (same clone as 28351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 28351-1-AP (same clone as 28351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 28351-1-AP (same clone as 28351-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PRKAR2B (28351-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

28351-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA PRKAR2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKAR2B fusion protein Ag28787
• Vollständiger Name: protein kinase, cAMP-dependent, regulatory, type II, beta
• Berechnetes Molekulargewicht: 418 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa
• GenBank-Zugangsnummer: BC075800
• Gene symbol: PRKAR2B
• Gene ID (NCBI): 5577
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.